Application of the Validated In Vitro Reconstructed Human Skin and Eye Models For Hazard Identification of the Chemical Mixtures

The international and national directives legislation requires to perfom validated in vitro alternative methods animal testing of chemicals mixtures for predictible precise evoluation toxicity human. regulatory agencies require the hazard assessment on skin ocular as reported by United Nations, Globally Harmonized System (UN GHS) Classification Labeling Chemicals. To address this need investigate usage methods, five representative chemical were prepared different compositions their was evaluated using EpiDerm™ SCT corrosion, SIT irritation, EpiOcular™ OCL eye irritation. tested 1 3 found corrosive irritant both eye. They identified category according UN GHS. 2, 4, 5 be neither nor GHS No Category. potential Reconstructed Human Epidermis Cornea-like Epithelial a replacement contributed significantly classify irritation/corrosion liquid solid comply with requirements. This study confirm that use assays instead conventional vivo test could very reliable accurate tool evaluate identification mixtures.